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Генетически модифицированные штаммы бактерий (GMStrains).

[Часть III]

Plasmids

Key:

1. Plasmid name
2. IMG accession number
3. Incompatibility group / Molecular weight
4. Phenotype
5. Host strain
6. Extra information: original host/properties/how derived or constructed
7. Reference(s) up to 2

Transposons

Key:

1. Transposon name
2. Origin
3. Molecular weight
4. Phenotype
5. IMG Accession number
6. Host strain and recombinant plasmid containing transposon
7. Host strain and :: transposon in chromosome
8. Reference(s)

   Tn1
   	RP1
   	5.0 kb
   	Ap
   	IMG-7163 ECO K12 JM83
   	pACYC184Tc::Tn1
   	Berg CM & Berg DE
   	in Microbiology-1981 Ed Schlessinger D
   	Washington 1981
   Tn9
   	P1Cm
   	2.6 kb
   	Cm
   	IMG-61084 ECO K12 CH111
   	RP1-Rep::Tn9
   	Callas MP & Miller JH
   	Cell 20:579-595
   Tn21
   	NR2(R100) from Shigella flexneri
   	19.7 kb
   	Hg Su Sm
   	IMG-61086 ECO K12 UB5201
   	pUB837 (pBR322::Tn21)
   	de la Cruz F & Grinsted J
   	J Bacteriol 151:222-228
   	Liebert et al
   	Microbiol&Mol Biol Rev 63:507-522
   Tn501
   	pVS1
   	8.2 kb
   	Hg
   	IMG-61092 ECO K12 UB5201
   	pUS982 (pBR322::Tn501)
   	IMG-61067
   	R388::Tn501
   	Bennet at al
   	Mol Gen Genet 159:101-106
   Tn701
   	R446b
   	8.5 kb
   	Tc
   	IMG-62721 ECO K12 HB101
   	pUC19::Tn701
   	IMG-62725 ECO K12 MKD135
   	pOX38gen::Tn701
   	Minakhina SV et al
   	Mol Microbiol 33:1059-1068
   Tn1721
   	pRSD1
   	11.4 kb
   	Tc
   	IMG-61087 ECO K12 UB5201
   	pJOE105 (pBR322D:1721)
   	Schmitt R et al
   	Mol Gen Genet 172:53-65
   Tn3401
   	Hg
   	IMG-6979 ECO K12 J53-1
   	R388::Tn3401
   	Foster TJ & Ginnity F
   	J Bacteriol 162:773-776
   Tn5036v1
   	Aeromonas sp.HU1-5 (New York,
   	river Hudson)
   	7.8 kb
   	Hg
   	IMG-786 ECO K12 AB1456
   	pRP138(RP1::Tn5036)
   	IMG Collection
   Tn5039
   	Unidentified Gram negative strain BW8
   	(USA, Bethesda, water)
   	20 kb
   	Hg
   	IMG-61505 ECO K12 HB101
   	pRP139 (RP1::Tn5039)
   	IMG Collection
   Tn5041
   	PS sp KHP41 (Kirgizia)
   	14876 bp
   	Hg
   	IMG-61423 ECO K12 HB101
   	pRP141 (RP1::Tn5041)
   	Kholodii GYa et al
   	Microbiology 143:2549-2556
   Tn5041B
   	PSF TC29-5 (Carpathian)
   	14.8 kb
   	Hg
   	IMG-7128 ECO K12 JF238
   	pRP1.241B (pRP1.2::Tn5041B)
   	Kholodii GYa et al
   	Genetika (in Russian) 36:459-469
   Tn5041C
   	PS sp TC29-1 (Carpathian)
   	17 kb
   	Hg
   	IMG-7127 ECO K12 JF238
   	pRP141C (RP1::Tn5041C)
   	Kholodii G Ya et al
   	Genetika (in Russian) 36:459-469
   Tn5041D
   	PS sp MU10-2 (East Lansing, USA)
   	21.5 kb
   	Hg
   	IMG-7112 ECO K12 HB101
   	pRP141D (RP1::Tn5041D)
   	Kholodii G Ya et al
   	Genetika (in Russian) 36:459-469
   Tn5042
   	PSF ED94-63 (Russia, Kolyma)
   	7 kb
   	Hg PM
   	IMG-7118 ECO K12 AB1456
   	pRP1.242 (pRP1.2::Tn5042)
   	IMG Collection
   Tn5044
   	XHC TAP44-3 (Russia, Kamchatka)
   	10840 bp
   	Hg (Ts)
   	IMG-61353 ECO K12 AB1456
   	pRP144 (RP1::Tn5044)
   	IMG-7176 ECO K12 HB101
   	pACYC184Tc::Tn5044
   	Minakhina SV et al
   	Mol Microbiol 33:1059-1068
   Tn5046
   	PS sp LS46-6 (Russia, Saratov)
   	10118 bp
   	Hg
   	IMG-788 ECO K12 H101
   	pRP146 (RP1::Tn5046}
   	IMG Collection
   Tn5050
   	PS sp LS45-3 (Saratov, Russia)
   	8 kb
   	Hg
   	IMG-61538 ECO K12 AB1456
   	pRP150 (RP1::Tn5050)
   	IMG Collection
   Tn5051
   	PSP HU1-6
   	12 kb
   	Hg
   	IMG-61465 ECO K12 JM83
   	pRP151(RP1::Tn5051)
   	IMG Collection
   Tn5053
   	XH sp KHW17 (Kirgizia, mercury mine)
   	8447 bp
   	Hg
   	IMG-61475 ECO K12 JM83
   	pRP153 (RP1::Tn5053)
   	IMG-2426 ECO K12 IF238
   	MKD1215::Tn5053
   	Insertion into position 1390675
   	(AEOOO231)
   	Kholodii GYa et al
   	Mol Biol 230:1103-1107
   	Kholodii GYa et al
   	Mol Microbiol 17 :1189-1200
   Tn5054
   	Unidentified Gram-negative strain
   	MU5-4 (USA, East Lansing, water)
   	8,4 kb
   	Hg
   	IMG-61539 ECO K12 AB1456
   	pRP154 (RP1::Tn5054)
   	IMG Collection
   Tn5055
   	Enterobacter sp. CH2-4
   	(USA, Chicago)
   	8.4 kb
   	Hg
   	IMG-61506 ECO K12 HB101
   	pRP155 (RP1::Tn5055)
   	IMG Collection
   Tn5056
   	Unidentified Gram-negative strain
   	LS32-1 (Moscow district, water)
   	13.7 kb
   	Hg PM
   	IMG-795 ECO K12 HB101
   	pRP156 (RP1::Tn5056)
   	IMG Collection
   Tn5057
   	ECO CH210 (chromosome) (USA, Chicago)
   	8.5 kb
   	Hg
   	IMG-791 ECO K12 JM83
   	cloned into pUC19
   	IMG Collection
   Tn5058 PS sp. ED23-33 (Russia, Kolyma)
   	12.3 kb
   	Hg PM
   	IMG-782 ECO K12 AB1456
   	pRP158 (RP1::Tn5058)
   	IMG-2427 ECO K12 IF238
   	IF238-571::Tn5058
   	IMG Collection
   Tn5060
   	PS sp A19-1 (Russia, Kolyma)
   	8.7 kb
   	Hg
   	IMG-7133 ECO K12 IF238
   	pRP160 (RP1::Tn5060)
   	IMG-7174 ECO K12 HB101
   	pACYC184::Tn5060
   	IMG-7183 ECO K12 HB101
   	pBR322::Tn5060
   	IMG Collection
   Tn5061
   	P.alcaligenes FA8-1 (Russia,
   	Kamchatka)
   	Hg
   	IMG-7101 ECO K12 AB1456
   	pRP161 (RP1::Tn5061)
   	IMG Collection
   Tn5062
   	Unidentified Gram-negative strain
   	MU5-25 (USA, East Lansing, water)
   	Hg
   	IMG-61540 ECO K12 AB1456
   	pRP162 (RP1::Tn5062)
   	IMG Collection
   Tn5070
   	Unidentified Gram-negative strain
   	BW-13 (USA, Bethesda, water)
   	6692 bp
   	Hg
   	IMG-61468 ECO K12 JM83
   	pRP170 (RP1::Tn5070)
   	IMG-7184 ECO K12 HB101
   	pKLH70.1 (pBR322::Tn5070)
   	IMG-7175 ECO K12 HB101
   	pACYC184::Tn5070
   	IMG Collection
   Tn5071
   	Unidentified Gram-negative strain
   	HU2-1 (USA, New York, water)
   	>25 kb
   	Hg
   	IMG-61466 ECO K12 JM83
   	pRP171 (RP1::Tn5071)
   	IMG Collection
   Tn5072
   	Unidentified Gram-negative strain
   	TC33-5 (Carpathian, mercury mine)
   	Hg
   	IMG-7104 ECO K12 JF238
   	pRP172 (RP!;;Tn5072)
   	IMG Collection

   
   
   
   

   Escherichia Coli Host Strains
   (suitable for use as plasmid recipients)

   E.coli K12

   
   AB1157: lac thr leu thi pro his arg str
   AB1456: argH metB his ilv str rif
   C600: thi-1 thr-1 leuB6 lacY1 tonA21 supE44
   C600 rk mk: C600 HsdR- HsdM-
   CH111: ade argH lac gal xyl thi-1 trpA36 recA tonA strA
   DH1: thi-1 gyrA96 hsdR17 supE44 endA relA1 recA1
   HB101: thi pro leu hsdR hsdM recA56 str
   HMS174: hsdR recA rif-r
   J53: met pro
   J53-1: met pro nal-r
   JF238: prototrophic nal-r
   JM83: ara D(lac- pro) rpsL f80DlacZDM15
   LE392: metB1 lacY1 galK2 trpR55 supE44 supF58 hsdR514
   MKD135: argH rif-r recA
   UB5201: met pro recA56 gyrA
   XXLI-Blue: thi lac supE44 relA1 gyrA96 endA1 recA1 hsdR17 (F'proAB+ lacIq D(lacZ)M15 Tn10

   
   

   E.coli B host strains

   BL21: gal hsdS
   BL21(DE3): gal hsdS (cI857 ind-1 Sam7 nin5 lacUV5-T7 gene)

   
   
   

   Abbreviations

   Plasmids & Transposons

   Property	Description
   Asa	Arsenate resistance
   Asi	Arsenite resistance
   Ap	Ampicillin resistance
   Cm	Chloramphenicol resistance
   Gm	Gentamicin resistance
   Hg	HgCl2 resistance
   Km	Kanamycin resistance
   PM	Phenyl mercuric acetate resistance
   Sm	Streptomycin resistance
   Sp	Spectinomycin resistance
   Su	Sulphamide resistance
   Tc	Tetracycline resistance
   TOL	Toluene degradation

   
   

   Codes for the names of the bacterial species

   CODE	NAME
   ACA	Acinetobacter calcoaceticus
   ECO	Escherichia coli
   EBC	Enterobacter cloacae
   PSF	Pseudomonas fluorescens
   PSP	Pseudomonas putida
   PS sp	Pseudomonas sp.
   XHC	Xanthomonas campestris
   XH sp	Xanthomonas sp.

   

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